Phytochemical screening and structural elucidation of secondary metabolites from picramnia sp. in the ecuadorian amazon
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Abstract
The species of the genus Picramnia are found throughout tropical America, evidencing a wide distribution. In addition, secondary metabolites that some of them exhibit have been described. In the province of Napo, located in the Ecuadorian Amazon, one of the species of this genus is found, whose metabolites have not yet been elucidated. In the present work, the objective was to extract and isolate secondary metabolites from this plant, as well as to characterize them using spectroscopic techniques. For this, the aerial parts of the Picramnia sp. were collected in the Jatun Sacha Biological Reserve. The dried and ground leaves were then macerated consecutively in different solvents, and some of the obtained extracts were partitioned and / or purified by Flash Column Chromatography. Both the dried leaves and the obtained extracts were subjected to qualitative tests to determine the presence of the metabolites of interest. The presence of anthraquinones, tannins, terpenoids, phenols and flavonoids was verified. Finally, β-sitosterol and chrysophanol were identified in purified fractions, which were elucidated using a combination of 1H NMR, 13C NMR spectroscopy and mass spectrometry. It is known that both molecules have various pharmacological activities, among which their antimicrobial activity stands out.
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