COMPARATIVE STUDY OF THE METHODS SPECTROPHOTOMETRIC AND POTENTIOMETRIC FOR QUANTITATIVE DETERMINATION OF TOTAL PHENOLS IN ECUADORIAN MEDICINAL PLANTS
Article Sidebar
Main Article Content
Abstract
In this study, the values of total phenol concentrations in aqueous extracts of ecuadorian medicinal plants, obtained using the spectrophotometric method developed by Folinn and Ciocalteau and by the potentiometric titration method, were compared. By the potentiometric method, the total phenols present in four medicinal plants (Ageratum conyzoides, Cnidoscolus chayamansa, Cynara scolymus y Taraxacum officinale) were quantified, which were selected for being representatives of the high, medium, intermediate, and low contents of total phenols of the twelve plants previously analyzed spectrophotometrically. The results of the quantification of total phenols by potentiometric titration had a high correlation (R2 = 0.987) with the values obtained by the spectrophotometric method, at concentrations of 0.70 to 24.0 mg/g. The use of potentiometry to quantify total phenols was environmentally friendly by using very dilute concentrations of reagents and replacing the toxic substances commonly used in spectrophotometric methods.
Downloads
Article Details
- The authors agree to respect the academic information of other authors, and to assign the copyrights to the journal infoANALÍTICA, so that the article can be edited, published and distributed.
- The content of the scientific articles and the publications that appear in the journal is the exclusive responsibility of their authors. The distribution of the articles published in the infoANALÍTICA Journal is done under a Creative Commons Reconocimiento-CompartirIgual 4.0 Internacional License.
References
Blainski, A., Lopes G. & Palazzo de Mello, J. (2013). Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules, 18(6), 6852-6865. doi:10.3390/molecules18066852
Bravo, L. (1998). Poliphenol: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews, 56, 317-333. doi: 10.1111/j.1753-4887.1998.tb01670.x.
De Beer, D., Harbertson, J.F., Kilmartin, P.A., Roginsky, V., Barsukova, T., Adams, D.O., & Waterhouse, A.L. (2004). Phenolics: A comparison of diverse analytical methods. American Journal of Enology and Viticulture, 55, 389-400.
De Leite, K.C.S., Garcia, L.F., Lobón, G.S. & et al. (2018). Antioxidant activity evaluation of dried herbal extracts: an electroanalytical approach. Brazilian Journal of Pharmacognosy, 28, 325-332. https://doi.org/10.1016/j.bjp.2018.04.004.
Duthie, G.G., Duthie, S.J., & Kyle, J.A.M. (2000). Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutrition Research Reviews, 13(1),79-106. DOI: 10.1079/095442200108729016.
Elbehery, N.H.A., Amr, A.E.-G.E., Kamel, A.H., Elsayed, E.A. & Hassan, S.S. (2019). Novel potentiometric 2,6-dichlorophenolindo-phenolate (DCPIP) membrane-sased Sensors: Assessment of their Input in the determination of total phenolics and ascorbic acid in beverages. Sensors, 19(9), 2058. https://doi.org/10.3390/s19092058.
Escarpa, A. & González, M.C. (2001) Approach to the content of total extractable phenolic compounds from different food samples by comparison of chromatographic and spectrophotometric methods. Analytica Chimica Acta, 427, 119-127. http://dx.doi.org/10.1016/S0003-2670(00)01188-0.
Folinn, C., & Ciocalteau, V. (1927). Tyrosine and tryptophan determination in proteins. The Journal of the biological Chemistry, 73, 627-650.
Ghaima, K. K., Hashim, N. M., & Ali, S. A. (2013). Anti¬bacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale). Journal of Applied Pharmaceutical Science, 3, 96-99. doi:10.7324/JAPS.2013.3518
Hoyos, J., Vázquez, M., & Contreras-Calderón, J. (2017). Electrochemical methods as a tool for determining the antioxidant capacity of food and beverages: A review. Food Chemistry, 221, 1371- 1381. https://doi.org/10.1016/j.foodchem.2016.11.017
Hudec, J, Ria Burdovaä M., Kobida, L. Komora L., Macho V., Kogan G., Turianica I., & et al. (2007). Antioxidant Capacity Changes and Phenolic Profile of Echinacea purpurea, Nettle (Urtica dioica L.), and Dandelion (Taraxacum officinale) after Application of Polyamine and Phenolic Biosynthesis Regulators. Journal of Agricultural and Food Chemistry, 55(14), 5689-96. doi:10.1021/jf070777c
Huma F, Jaffar M, & Masud K. (1999). A Modified potentiometric method for the estimation of phenol in aqueous systems. Turkish Journal of Chemistry, 23, 415-422.
Liu, J., Yong, H., Yao, X., Hu, H., Yun, D., & Xiao, L. (2019). Recent advances in phenolic–protein conjugates: synthesis, characterization, biological activities and potential applications RSC Advances, 9, 35825-35840. https://doi.org/10.1039/C9RA07808H
Makkar, H. P., Norvsambuu, T., Lkhagvatseren, S., & Becker, K. (2009). Plant secondary metabolites in some medicinal plants of Mongolia used for enhancing ani-mal health and production. Tropicultura, 27, 159-167. doi: 10.3390/genes9060309.
Martínez- Valverde, I., Periago, M., & Ros, G. (2000). Significado nutricional de los compuestos fenólicos de la dieta. Archivos Latinoamericanos de Nutrición, 50(1), 5-18.
Mizzi, L., Chatzitzika, C., Gatt, R., & Valdramidis, V. (2020). HPLC analysis of phenolic compounds and flavonoids with overlapping peaks. Food technology and biotechnology, 589(1), 12-19. https://doi.org/10.17113/ftb.58.01.20.6395.
Mota, F., Queimada, A., Pinho, S., & Macedo. E. (2008). Aqueous solubility of some natural phenolic compounds. Industrial and Engineering Chemistry Research, 47(15), 5182-5189. https://doi.org/10.1021/ie071452o
Nijveldt, R.J., Van Nood, E., Van Hoorn, D.E.C., Boelens, P.G., & Van Norren, K. (2001). Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74, 418-425. https://doi.org/10.1093/ajcn/74.4.418
Rodríguez-Méndez, M., Apetrei, C., & De Saja, J. (2008). Evaluation of the polyphenolic content of extra virgin olive oils using an array of voltammetric sensors. Electrochimica Acta, 53, 5867-5872. https://doi.org/10.1016/j.electacta.2008.04.006
Salehi, B., Azzini E., Zucca P., Varoni E., Anil Kumar N., Luciana Dini L. & et al. (2020). Plant-derived bioactives and oxidative stress-related disorders: A key trend towards healthy aging and longevity promotion. Applied Science, 10, 947; doi:10.3390/app10030947.
Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., & Ercisli, S. (2009). Total phenolic content, antioxi¬dant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences, 22, 102-106.
Serrano, J., Puupponen-Pimiä, R., Dauer, A., Aura, A.M., & Saura-Calixto, F. (2009). Tannins: Current knowledge of food sources, intake, bioavailability, and biological effects. Molecular Nutrition & Food Research, 53, 310-329. DOI: 10.1002/mnfr.200900039
Singleton, V.L., Orthofer, R., & Lamuela-Raventós, R.M (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Sun, Z., Zhang, Y., Xu, X., Wang, M., & Kou L. (2019). Determination of the total phenolic content in wine samples using potentiometric method based on permanganate ion as an indicator. Molecules, 24(18), 3279. https://doi.org/10.3390/molecules24183279.
Velásquez A. (2004). Extracción de taninos presentes en el banano verde. Revista Lasallista de Investigación, 1(2), 17-22.
Wang, J. & Li, R. (1989). Highly stable voltametric measurements of phenolic compounds at poly(3-methylthiophene)-coated glassy carbon electrodes. Analytical Chemistry, 61, 2809-2811. https://doi.org/10.1021/ac00199a025
World Health Organization (WHO) (2011). Quality control methods for medicinal plant materials. WHO Pres. Geneva. p 1-4, 26-31.