DETERMINATION OF THE ANTIOXIDANT CAPACITY IN VITRO OF A CREAM MADE WITH ISOLATED CANNABIDIOL EXTRACT OF Cannabis sativa L. (HEMP)
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Abstract
The present investigation determined the antioxidant activity of a facial antioxidant cream, made with Cannabidiol (CBD) isolated extract. To establish the antioxidant capacity, the 2,2-Diphenyl-1-Picrylhydrazil (DPPH) method was used to aply a triplicate design, in addition, ascorbic acid (AA) was used as a positive control. Antioxidant capacity of the extract and the cream formulated with the CBD extract were terminated spectrometrically, the wavelength used was 517 nm. Seven creams with different CBD concentrations, ranging between 0,5 and 10%, were developed. The percentage of inhibition of the DPPH radical of the CBD extract was 91.93% and for the cream it was 83,00% at the highest concentration of 10,00 mg/mL in both cases. Likewise, the IC50 values of the CBD extract and the cream were established with values of 1.8803±0.188 mg/mL and 2.340±0.034 mg/mL respectively. The present study promotes further investigation of a correct use of CBD isolate extract in cosmetic products with antioxidant benefits.
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